![]() ![]() TEM instruments have multiple operating modes including conventional imaging, scanning TEM imaging (STEM), diffraction, spectroscopy, and combinations of these. TEMs find application in cancer research, virology, and materials science as well as pollution, nanotechnology and semiconductor research, but also in other fields such as paleontology and palynology. ![]() Transmission electron microscopy is a major analytical method in the physical, chemical and biological sciences. This enables the instrument to capture fine detail-even as small as a single column of atoms, which is thousands of times smaller than a resolvable object seen in a light microscope. Transmission electron microscopes are capable of imaging at a significantly higher resolution than light microscopes, owing to the smaller de Broglie wavelength of electrons. The image is then magnified and focused onto an imaging device, such as a fluorescent screen, a layer of photographic film, or a sensor such as a scintillator attached to a charge-coupled device. An image is formed from the interaction of the electrons with the sample as the beam is transmitted through the specimen. The specimen is most often an ultrathin section less than 100 nm thick or a suspension on a grid. Transmission electron microscopy ( TEM) is a microscopy technique in which a beam of electrons is transmitted through a specimen to form an image. Operating principle of a transmission electron microscope Technique in microscopy A TEM image of a cluster of poliovirus. ![]()
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